ASTM D5465-93(2012)

Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

STANDARD published on 1.6.2012

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The information about the standard:

Designation standards: ASTM D5465-93(2012)
Note: WITHDRAWN
Publication date standards: 1.6.2012
SKU: NS-31337
The number of pages: 4
Approximate weight : 12 g (0.03 lbs)
Country: American technical standard
Category: Technical standards ASTM

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The category - similar standards:

Microbiology of water

Annotation of standard text ASTM D5465-93(2012) :

Keywords:

calculating results, counting colonies, membrane filter methods, pour plate methods, reporting results, rounding off, significant figures, spread plate methods, verification of counts, ICS Number Code 07.100.20 (Microbiology of water)

Additional information

Significance and Use

These practices provide a uniform set of counting, calculating, and reporting procedures for ASTM test methods in microbiology.

	Section
ACounting Colonies on Membrane Filters	4
BCounting Colonies on Pour Plates	5
CCounting Colonies on Spread Plates	6

The counting rules provide a best attainable estimate of microorganisms in the sample, since the samples cannot be held and reanalyzed at a later date.

1. Scope

1.1 These practices cover recommended procedures for counting colonies and reporting colony-forming units (CFU) on membrane filters (MF) and standard pour and spread plates.

1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

2. Referenced Documents

D5259-19	Standard Test Method for Isolation and Enumeration of Enterococci from Water by the Membrane Filter Procedure
D1129-13(2020)e2	Standard Terminology Relating to Water (Includes all amendments and changes 2/14/2024).
9215	Heterotrophic Plate Count Available online from http://standardmethods.org/store/ProductView.cfm?ProductID=102, or from American Public Health Association (APHA), Standard Methods for the Examination of Water and Wastewater, 800 I Street, NW Washington, DC 20001, http://www.apha.org.
E2563-23	Standard Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method
D6974-20	Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels´Filtration and Culture Procedures
D6161-19	Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
D5392-19	Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure