Standard Test Methods for Evaluation of Inactivators of Antimicrobial Agents
STANDARD published on 1.4.2013
Designation standards: ASTM E1054-08(2013)
Publication date standards: 1.4.2013
The number of pages: 7
Approximate weight : 21 g (0.05 lbs)
Country: American technical standard
Category: Technical standards ASTM
antimicrobial agents, antimicrobial effectiveness evaluations, inactivation, neutralization, neutralizer toxicity, ICS Number Code 11.080.20 (Disinfectants and antiseptics), 71.100.35 (Chemicals for industrial and domestic desinfection purposes)
|Significance and Use|
5.1 The effectiveness of antimicrobial agents incorporated into disinfectants, sanitizers, and antiseptics is measured by their ability to kill microorganisms within a specified contact time. Hence, accurate determination of antimicrobial effectiveness requires complete and immediate inactivation (neutralization) of the antimicrobial agent. Inefficient or incomplete neutralization will permit killing or inactivation of microorganisms to continue beyond the experimental exposure time, resulting in an overestimation of antimicrobial activity.
5.2 The neutralization methods commonly used in antimicrobial effectiveness evaluations are chemical inactivation, dilution, and filtration. All critical parameters of an antimicrobial effectiveness evaluation—for example, media, equipment, microorganism(s), and temperature of solutions—must be duplicated when evaluating a neutralization procedure to be used.
5.3 The neutralization evaluation must include at least three replications (five replications in Section 5.4 A limitation of these evaluation procedures is that they use microorganisms that have not been exposed to an antimicrobial agent. Under experimental conditions, cells exposed to neutralization procedures are likely to be damaged to different degrees by the antimicrobial agent. Sublethal injury may be a factor in recovery, and the role of the neutralization procedure in recovery of injured organisms should be examined. This method is not intended to assess injured organism recovery.
1.1 These test methods are used to determine the effectiveness of procedures and agents for inactivating (neutralizing, quenching) the microbicidal properties of antimicrobial agents, and to ensure that no components of the neutralizing procedures and agents, themselves, exert an inhibitory effect on microorganisms targeted for recovery.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
|2. Referenced Documents|
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