Standard Practice for Using Octanol-Water Partition Coefficient to Estimate Median Lethal Concentrations for Fish Due to Narcosis
STANDARD published on 1.10.2014
Designation standards: ASTM E1242-97(2014)
Publication date standards: 1.10.2014
The number of pages: 2
Approximate weight : 6 g (0.01 lbs)
Country: American technical standard
Category: Technical standards ASTM
ICS Number Code 07.080 (Biology. Botany. Zoology)
|Significance and Use|
5.1 This procedure can be used to limit the need for screening tests prior to performing a test for estimating the LC50 of a non-reactive and non-electrolytic chemical to the fathead minnow. By eliminating the screening test, fewer fish need be tested. The time used for preparing and performing the screening test can also be saved. The value obtained in this procedure can be used as the preliminary estimate of the LC50 in a full-scale test.
5.2 Estimates can be used to set testing priority of groups of non-reactive and non-electrolytic chemicals.
5.3 If the estimated value is more than 0.3 times the experimental value, the mechanism of action is probably narcosis. If less, the effect concentration is considered to reflect a different mechanism of action.
5.4 This practice estimates a maximum LC50, that is, non-reactive and non-electrolytic chemicals are at least as toxic as the practice predicts, but may have a lower LC50 if acting by a more specific mechanism. Data on a chemical indicating a lower toxicity than predicted should be considered suspect or an artifact because of limited solubility of the test material.
1.1 This practice covers a procedure for
estimating the fathead minnow (Pimephales promelas) 96-h LC50 of
nonreactive (that is, covalently bonded without unsaturated
residues) and nonelectrolytic (that is, require vigorous reagents
to facilitate substitution, addition, replacement reactions and are
non-ionic, non-dissociating in aqueous solutions) organic chemicals
acting solely by narcosis, also referred to as Meyer-Overton
toxicity relationship.1.2 This procedure is accurate for
organic chemicals that are toxic due to narcosis and are
non-reactive and non-electrolytic. Examples of appropriate
chemicals are: alcohols, ketones, ethers, simple halogenated
aliphatics, aromatics, and aliphatic substituted aromatics. It is
not appropriate for chemicals whose structures include a potential
toxiphore (that structural component of a chemical molecule that
has been identified to show mammalian toxicity, for example CN is
known to be reponsible for inactivation of enzymes,
Guide for Conducting Acute Toxicity Tests
on Test Materials with Fishes, Macroinvertebrates, and
Amphibians Guide for Assessing the Hazard of a
Material to Aquatic Organisms and Their Uses Terminology Relating to Biological
Effects and Environmental Fate Test Method for Partition Coefficient
(N-Octanol/Water) Estimation by Liquid Chromatography
Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
Terminology Relating to Biological Effects and Environmental Fate
Test Method for Partition Coefficient (N-Octanol/Water) Estimation by Liquid Chromatography
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