Standard Guide for Conducting Short-Term Chronic Toxicity Tests with Echinoid EmbryosTranslate name
STANDARD published on 15.1.2021
Designation standards: ASTM E1563-21
Publication date standards: 15.1.2021
The number of pages: 23
Approximate weight : 69 g (0.15 lbs)
Country: American technical standard
Category: Technical standards ASTM
acute toxicity, Arbacia punctulata, Dendraster excentricus, echinoid, EC50 test, embryo development, marine water quality, pluteus larva, sand dollar, sea urchin, sediment test, short-term chronic toxicity, static toxicity test, Strongylocentrotus droebachiensis, Strongylocentrotus purpuratus, toxicity, toxicology,, ICS Number Code 07.080 (Biology. Botany. Zoology),07.100.20 (Microbiology of water),13.020.40 (Pollution, pollution control and conservation)
|Significance and Use|
5.1ï¿½An acute toxicity test is conducted to assess effects of a short-term exposure of organisms to a test material under specific experimental conditions. An acute toxicity test does not provide information concerning whether delayed effects will occur and typically evaluates effects on survival. A chronic test is typically longer in duration and includes a sublethal endpoint to assess effects on a population that might occur beyond the exposure period. Because the echinoderm embryo development test includes a sublethal endpoint, but is also short in duration, these tests are considered to be short-term chronic tests, consistent with EPA guidance.
5.2ï¿½Because embryos and larvae are usually assumed to be the most sensitive life stages of these echinoid species, and because some of these species are commercially and recreationally important, the results of these tests are often considered to be a good indication of the acceptability of pollutant concentrations to saltwater species in general. The results of these toxicity tests are often assumed to be an important consideration when assessing the hazard of materials to other saltwater organisms (see Guides and ) or when deriving water quality criteria for saltwater organisms 5.3ï¿½The results of short-term chronic toxicity tests might be used to predict effects likely to occur to aquatic organisms in field situations as a result of exposure under comparable conditions, except that toxicity to benthic species might depend on sorption or settling of the test material onto the substrate.
5.4ï¿½The results of short-term chronic tests might be used to compare the sensitivities of different species and the acute toxicities of different test materials, and to determine the effects of various environmental factors on the results of such tests.
5.5ï¿½The results of short-term chronic toxicity tests might be useful for studying the biological availability of, and structure-activity relationships between, test materials.
5.6ï¿½The results of any toxicity tests will depend on temperature, composition of the dilution water, condition of the test organisms, and other factors.
5.7ï¿½Results of short-term chronic toxicity tests might be used to predict effects likely to occur to aquatic organisms exposed to suspended particulates of dredged sediments disposed through the water column.
5.8ï¿½Results of short-term chronic toxicity tests might be used to predict effects likely to occur to aquatic organisms exposed to bedded whole sediments.
1.1ï¿½This guide covers procedures for obtaining laboratory data concerning the short-term chronic effects of a test material on echinoderm embryos and the resulting larvae (sea urchins and sand dollars) during static 48- to 96-h exposures. These procedures have generally been used with U.S. East Coast (Arbacia punctulata and Strongylocentrotus droebachiensis ) 1.2ï¿½Other modifications of these procedures might be justified by special needs or circumstances. Although using procedures appropriate to a particular species or special needs and circumstances is more important than following prescribed procedures, the results of tests conducted by using unusual procedures are not likely to be comparable with those of many other tests. The comparison of results obtained by using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting tests starting with embryos of echinoids.
1.3ï¿½These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide ), leachates, oils, particulate matter, surface waters, effluents, and sediments ( ). Renewal tests might be preferable to static tests for materials that have a high oxygen demand, are highly volatile, are rapidly transformed biologically or chemically in aqueous solution, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test.
1.4ï¿½Results of short-term chronic toxicity tests with echinoid embryos should usually be reported as the 50â€‰% effect concentration (EC50) based on the total abnormally developed embryos and larvae. In some situations, it might only be necessary to determine whether a specific concentration is toxic to embryos or whether the EC50 is above or below a specific concentration.
1.5ï¿½This guide is arranged as follows:
1.6ï¿½The values stated in SI units are to be regarded as the standard.
1.7 1.8ï¿½This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
|2. Referenced Documents|
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