Standard Guide for Cell Culture Analysis with SIMSTranslate name
STANDARD published on 1.12.2020
Designation standards: ASTM E1881-12(2020)
Publication date standards: 1.12.2020
The number of pages: 3
Approximate weight : 9 g (0.02 lbs)
Country: American technical standard
Category: Technical standards ASTM
SIMS ,, ICS Number Code 07.100.10 (Medical microbiology)
|Significance and Use|
5.1ï¿½The presence of cell growth medium complicates a direct analysis of cells with SIMS. Attempts to wash out the nutrient medium results in the exposure of cells to unphysiological reagents that may also alter their chemical composition. This obstacle is overcome by using a sandwich freeze-fracture method 5.2ï¿½The procedure described here has been successfully used for imaging Na+ and K+ ion transport (, calcium alterations in stimulated cells )5.3ï¿½The procedure described here is amenable to a wide variety of cell cultures and provides a way for studying the response of individual cells for chemical alterations in the state of health and disease and localization of isotopically-labeled molecules and theraputic drugs in cell culture models.
1.1ï¿½This guide provides the Secondary Ion Mass Spectrometry (SIMS) analyst with a cryogenic method for analyzing individual tissue culture cells growing in vitro. This guide is suitable for frozen-hydrated and frozen-freeze-dried sample types. Included are procedures for correlating optical, laser scanning confocal and secondary electron microscopies to complement SIMS analysis.
1.2ï¿½This guide is not suitable for cell cultures that do not attach to the substrate.
1.3ï¿½This guide is not suitable for any plastic embedded cell culture specimens.
1.4ï¿½This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.5ï¿½This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
|2. Referenced Documents|
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