Standard ČSN EN 12821 1.12.2012 preview

ČSN EN 12821 (560047)

Foodstuffs - Determination of vitamin D by high performance liquid chromatography - Measurement of cholecalciferol (D3) or ergocalciferol (D2)



STANDARD published on 1.12.2012


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The information about the standard:

Designation standards: ČSN EN 12821
Classification mark: 560047
Catalog number: 92039
Publication date standards: 1.12.2012
SKU: NS-141242
The number of pages: 24
Approximate weight : 72 g (0.16 lbs)
Country: Czech technical standard
Category: Technical standards ČSN

Annotation of standard text ČSN EN 12821 (560047):

Tato norma specifikuje metodiku pro stanovení vitamínu D v potravinách. Vitamín D je po zmýdelnění z potraviny extrahován vhodným rozpouštědlem. Stanovení vitamínů D3 a D2 ve vhodném extraktu vzorku je provedeno semipreparativní HPLC na normální fázi s následnou analytickou HPLC na reverzní fázi. Vitamín D je detekován spektrofotometricky v UV oblasti. Pokud se stanovuje vitamín D3, pak je vitamín D2 použit jako interní standard. Jestliže se stanovuje vitamín D2, pak je vitamín D3 použit jako interní standard

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