ČSN EN 14352 (560024)

Foodstuffs - Determination of fumonisin B1 and B2 in maize based foods - HPLC method with immunoaffinity column clean up



STANDARD published on 1.12.2004


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The information about the standard:

Designation standards: ČSN EN 14352
Classification mark: 560024
Catalog number: 71899
Publication date standards: 1.12.2004
SKU: NS-145151
The number of pages: 16
Approximate weight : 48 g (0.11 lbs)
Country: Czech technical standard
Category: Technical standards ČSN

The category - similar standards:

Cereals, pulses and derived products

Annotation of standard text ČSN EN 14352 (560024):

Tato norma specifikuje metodu pro stanovení fumonisinu B1 (FB1) a fumonisinu B2 (FB2) v potravinách na bázi kukuřice. Fumonisiny jsou extrahovány ze vzorku směsí vody, methanolu a acetonitrilu. Zfiltrovaný extrakt je přečištěn na imunoafinitní kolonce a fumonisiny jsou eluovány methanolem. Extrakt je odpařen, zbytek po odpaření je rozpuštěn ve směsi acetonitrilu a vody. Poté je přidán o-phthaldialdehyd/2-merkaptoethanol (OPA/MCE) pro vytvoření fluoreskujících derivátů fumonisinu, které jsou analyzovány vysokoúčinnou kapalinovou chromatografií na reverzní fázi (RP-HPLC) s fluorescenční detekcí

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