ČSN EN 16006 (467027)

Animal feeding stuffs - Determination of the Sum of Fumonisin B1 & B2 in compound animal feed with immunoaffinity clean-up and RP-HPLC with fluorescence detection after pre- or post-column derivatisation



STANDARD published on 1.10.2012


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The information about the standard:

Designation standards: ČSN EN 16006
Classification mark: 467027
Catalog number: 91510
Publication date standards: 1.10.2012
SKU: NS-147556
The number of pages: 20
Approximate weight : 60 g (0.13 lbs)
Country: Czech technical standard
Category: Technical standards ČSN

The category - similar standards:

Animal feeding stuffs

Annotation of standard text ČSN EN 16006 (467027):

Tato evropská norma se používá pro stanovení fumonisinu B1 a B2 (FB1 a FB2) ve směsi krmiva v úrovních začínajících od 3 mg/kg až do 16 mg/kg. FB1 a FB2 se extrahují ze zkoušeného materiálu pomocí 50% roztoku methanolu ve fosfátovém tlumivém roztoku (PBS). Poté se extrakt zředí pomocí PBS a přečistí pomocí imunoafinitních kolonek (IAC). FB1 a FB2 se eluují z IAC pomocí methanolu a následně vody. Po nastavení objemu se eluát přímo nastříkne do HPLC a FB1 a FB2 se stanoví na základě jejich fluorescence buď před- nebo postkolonovou derivatizací

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