ČSN EN 18057 (569920)

Food authenticity - Quantitation of roe deer DNA relative to mammalian DNA in meat and meat products by realtime PCR

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STANDARD published on 1.11.2025


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The information about the standard:

Designation standards: ČSN EN 18057
Classification mark: 569920
Catalog number: 522151
Publication date standards: 1.11.2025
SKU: NS-1245470
The number of pages: 20
Approximate weight : 60 g (0.13 lbs)
Country: Czech technical standard
Category: Technical standards ČSN

The category - similar standards:

Meat and meat products

Annotation of standard text ČSN EN 18057 (569920):

This document specifies a real-time PCR procedure for the quantitation of the amount of roe deer DNA relative to total mammalian DNA in meat and meat products.

Results of this roe deer assay are expressed in terms of roe deer (Capreolus capreolus) haploid genome copy numbers relative to total mammalian haploid genome copy numbers. The content of roe deer can also be expressed as mass fraction in % using gravimetrically prepared calibration material from meat mixtures or model samples.

The method has been previously validated in a collaborative study and applied to DNA extracted from samples that consist of raw roe deer meat in a raw pig meat background as well as raw and boiled sausages.

The limit of detection of the roe deer PCR has been determined experimentally to be at least 5 target gene copies or at least 0,1 % roe deer.

The compliance assessment process is not part of this document

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