ČSN EN ISO 13885-3 (673038)

Gel permeation chromatography (GPC) - Part 3: Water as eluent

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STANDARD published on 1.3.2022


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The information about the standard:

Designation standards: ČSN EN ISO 13885-3
Classification mark: 673038
Catalog number: 513726
Publication date standards: 1.3.2022
SKU: NS-1050133
The number of pages: 36
Approximate weight : 108 g (0.24 lbs)
Country: Czech technical standard
Category: Technical standards ČSN

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Annotation of standard text ČSN EN ISO 13885-3 (673038):

This document specifies the determination of the molar-mass distribution and the average molar mass values Mn (number average) and Mw (weight average) of polymers that are soluble in water by gel permeation chromatography (GPC). NOTE - Also known as size exclusion chromatography (SEC). This method is applicable to neutral polymers and polyanions (e.g. polycarboxylates, polysaccharides, fully hydrolyzed polyvinyl alcohols and high-molecular polyethylene oxides). It is not applicable to polycations [e.g. polyvinylpyrrolidone, polyvinylpyridine, salts of poly(diallyl-N,N-dimethyl-azacyclopentane), chitosan]. Despite good solubility in the mobile phase and even though the chromatograms obtained show good repeatability, it is possible that this method cannot be used with certain polymer types because of specific interactions (e.g. adsorption) within the sample/eluent/column system (see also Clause 12). The conditions specified in this document are not applicable to the GPC analysis of polymer samples with Mw values greater than 106 g/mol and/or polymers with elution limits outside the calibration range (see 7.6 and Annex C). This document includes no correction methods (e.g. for the elimination of peak broadening). If absolute molar mass values are required, an absolute method (e.g. membrane osmometry for Mn or light scattering for Mw) can be used

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