ČSN EN ISO 6579-4 (560088)

Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 4: Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)

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STANDARD published on 1.7.2025


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The information about the standard:

Designation standards: ČSN EN ISO 6579-4
Classification mark: 560088
Catalog number: 521384
Publication date standards: 1.7.2025
SKU: NS-1224624
Approximate weight : 144 g (0.32 lbs)
Country: Czech technical standard
Category: Technical standards ČSN

Annotation of standard text ČSN EN ISO 6579-4 (560088):

This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium (1,4,[5],12:i:-) lacking the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression.

The method is applicable for:

  • - differentiation of the isolate under analysis between monophasic Salmonella Typhimurium and the monophasic variant of another Salmonella on-Typhimurium serovar that has the same antigenic formula;
  • - identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium.

This document is applicable for the analysis of a pure culture belonging to the genus Salmonella, isolated from:

  • - products intended for human consumption;
  • - products intended for animal feeding;
  • - environmental samples in the area of food and feed production and handling;
  • - samples from the primary production stage.

This document can also be applied in other domains for identification of monophasic Salmonella Typhimurium (e.g. environmental, human health, animal health)

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