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Standard ČSN ISO 8870 1.1.2007 preview

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ČSN ISO 8870 (560641)

Milk and milk-based products - Detection of thermonuclease produced by coagulase-positive staphylococci



STANDARD published on 1.1.2007


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The information about the standard:

Designation standards: ČSN ISO 8870
Classification mark: 560641
Catalog number: 77626
Publication date standards: 1.1.2007
SKU: NS-180597
The number of pages: 12
Approximate weight : 36 g (0.08 lbs)
Country: Czech technical standard
Category: Technical standards ČSN

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Milk and milk products in general

Annotation of standard text ČSN ISO 8870 (560641):

Tato česká norma specifikuje metodu průkazu termostabilní DNázy (termonukeázy) produkované koaguázopozitivními stafylokoky v mléce a výrobcích na bázi mléka. Zkoušený výrobek se upraví popsaným postupem a stanovené množství se vnese do jamky v agaru s DNA a toluidinovou modří O. Souběžně se zkouší pozitivní kontrola (příslušně upravená kultura termonukleázopozitivního kmene Staphylococcus aureus). Růžová okrouhlá zóna o šířce 1 mm nebo více (srovnává se s pozitivní kontrolou) vytvořená v okolí jamky indikuje přítomnost termonukleázy. Barevná reakce (z modré na růžovou) je způsobena metachromatickými vlastnostmi toluidinové modři O. Pozitivní termonukleázová zkouška provedená podle této normy indikuje, že koagulázopozitivní stafylokoky dosáhly počtů 106/g nebo vyšších. V případě tvorby enterotoxinu mohou takové počty vést k jeho koncentraci, která je dostatečně vysoká, aby způsobila onemocnění. Proto výrobky pozitivní v termonukleázové zkoušce mají být zkoušeny na přítomnost enterotoxinů.

Preview of the standard ČSN ISO 8870 (560641)

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