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ČSN P CEN/TS 17883 (838047)
Environmental characterization of leachates from waste and soil using reproductive and toxicological gene expression in Daphnia magna
Translate name
STANDARD published on 1.7.2023
The information about the standard:
Designation standards: ČSN P CEN/TS 17883
Classification mark: 838047
Catalog number: 516984
Publication date standards: 1.7.2023
SKU: NS-1147253
The number of pages: 28
Approximate weight : 84 g (0.19 lbs)
Country: Czech technical standard
Category: Technical standards ČSN
The category - similar standards:
Wastes in generalExamination of biological properties of waterOther standards related to soil quality
Annotation of standard text ČSN P CEN/TS 17883 (838047):
This document specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to quantify the abundance of specific mRNA molecules extracted from Daphnia magna. The method allows the identification of molecular responses to exposures for potentially toxic substances through the analysis of the abundance of specific mRNA molecules. In this document, the central genes involved in reproductive and toxic responses are included. The present method allows for rapid, robust and sensitive detection of molecular responses and can be used to analyse the toxic effects of water leachates from soil and waste. The method gives information of the concentration of a substance or test-liquid at which toxic effects begin to occur prior to observations of reproductive or toxic effects at higher levels of organization, which reduces the need for the use of safety factors in toxicity assessment. The method is useful in several types of risk assessment. In this document, the genes studied are appropriate for the assessment of the risks when recycling materials and for the classification of waste, but the method can be adapted to other types of risk assessment by including other genes
Preview of the standard ČSN P CEN/TS 17883 (838047)
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